Sensitivity of periodontal-pathological microflora and Candida fungi to dual probiotic

Abstract

Relevance. Treatment of Candida-associated periodontitis presents certain difficulties. It is connected with the reduction of the efficacy of antimycotic medications and antibiotics due to the development of resistance to them. At the same time, frequent use of antibiotics leads to population sensitization, and antimycotic drugs possess a set of side effects. In this connection, medications – probiotics from spore-forming Bacillus bacteria are of great interest, possessing a wide spectrum of antagonist activity concerning opportunistic pathogenic bacteria and fungi. These probiotics can produce a complex health improving action to the whole organism. Purpose. The of the research is to study the sensitivity of Candida fungi and periodontal-pathogenic microflora to dual probiotic, including Bacillus subtilis 11В and Bacillus licheniformis 31. Materials and methods. Material included 4 referent Candida fungus strains (C. albicans 001, C. albicans №СТС885-653, C. stellatoidea АТСС 10264, C. Krusei Harv АТСС 6259), 6 clinical isolates (C. albicans, С.Krusei, С. stellatoidea, C. tropicalis, C.glabrata, C.guilliermondi), as well as test cultures and clinical strains of periodontal-pathogenic microflora to dual probiotic. Sensitivity to dual probiotic with the determination of minimal suppressing concentration was conducted using deferred antagonism method (diffusion method - perpendicular streak technique). Results. Results of the study showed that both Candida fungi and periodontal-pathogenic microflora are sensitive to dual probiotic. Conclusion . Thus, antagonistic activity of dual probiotic regarding referent strains and clinical isolates of Candida fungi, as well as test cultures and clinical strains of periodontal-pathogenic microbes allows to use it in a complex treatment of Candidaassociated periodontitis.