Abstract

Fermentation of chemicals from lignocellulose hydrolysate is an effective way to alleviate environmental and energy problems. However, fermentation inhibitors in hydrolysate and weak inhibitor tolerance of microorganisms limit its development. In this study, atmospheric and room temperature plasma mutation technology was utilized to generate mutant strains of Enterobacter cloacae and screen for mutants with high inhibitor tolerance to acid hydrolysate of corncobs. A highly inhibitor-tolerant strain, Enterobacter cloacae M22, was obtained after fermentation with non-detoxified hydrolysate, and this strain produced 24.32 g/L 2,3-butanediol and 14.93 g/L organic acids. Compared with that of the wild-type strain, inhibitor tolerance was enhanced twofold with M22, resulting in improvement of 2,3-butanediol and organic acid production by 114% and 90%, respectively. This work presents an efficient method to screen for highly inhibitor-tolerant strains and evidence of a novel strain that can produce 2,3-butanediol and organic acids using non-detoxified acid hydrolysate of corncobs.

Highlights

  • Fermentation of chemicals from lignocellulose hydrolysate is an effective way to alleviate environmental and energy problems

  • Strain and media The original strain used in this study was E. cloacae CICC 10011, which was purchased from the China Center of Industrial Culture Collection (CICC)

  • The results showed that sugar concentration had no significant effect on cell growth (Fig. 2), which proved that the sugars in hydrolysate have no inhibitory effect on cell growth within the range of concentration investigated

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Summary

Introduction

Fermentation of chemicals from lignocellulose hydrolysate is an effective way to alleviate environmental and energy problems. These inhibitors need to be removed when using dilute acid hydrolysate of lignocellulose as a substrate for 2,3-BDO fermentation. Using non-detoxified acid hydrolysate as a raw material for fermentation with inhibitor-tolerant strains seems attractive (Schirmer-Michel et al 2009; Varga et al 2004). The ARTP mutation technique was used to create a library of Enterobacter cloacae mutants to screen for strains with high tolerance to inhibitors in corncob acid hydrolysate.

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