Screening differentially expressed genes of gastric stromal tumors originated form the muscularis propria by gene chip technology

Abstract

Objective To screen the related gene expression in gastric muscularis propria tumor by gene chip assay, and to guide the accurate diagnosis and targeted therapygastric of gastric stromal tumors originated form the muscularis propria. Methods 6 cases of samples with gastric muscularis propria mesenchymal tumor and peritumoral tissue were treated by endoscopic treatment and laparoscopic surgery. cDNA was synthesized by reverse transcription with total RNA, then by in vitro transcription synthesised of biotin labeling cRNA that was hybridizationed with the Affymetrix mRNA expression profile chip, screened differentially expressed genes with bioinformatics analysis using PANTHER/KEGG database and Gene Ontology software. Results 3 293 differentially expressed genes were detected in Gastric stromal tumor tissue, including 2 588 up-regulated, and 705 down-regulated genes, especially in DPP10, ETV1, DKK4, CXCL14, MT1M gene expression significantly. These genes were involved in multiple signaling pathways related to tumor, such as Wnt pathway, Rap1 pathway, ECM receptor interaction, Ras signaling pathway, P13K-AKT signaling pathway, and cell adhesion. Conclusion There are significant differences of gene expression between gastric stromal tumor and peritumoral tissue, the aitered expression of DPP10, ETV1, DKK4, CXCL14 and MT1M might be involved in the regulation of gastric stromal tumor, rarely reported before in the literature, and might be new molecular markers or treatment targets. To conduct in-depth study of these genes and related regulation pathways, will contribute to the diagnosis, treatment and prognosis of gastric stromal tumor. Key words: Gastric muscularis propria mesenchymal tumor; Gene chip; Differentially expressed genes