Abstract
Residual brewing yeast is one of the main solid wastes in brewing. Using residual brewing biomass as a source of biologically active substances is an important way of recycling these brewing by-products. According to the literature S. cerevisiae is considered as the promising source of polysaccharides, particularly beta-glucans. Beta-glucans are structural polysaccharides of the yeast cell and perform immune stimulating properties. At the same time, there is too little information about the content of these polysaccharides in brewing yeast of the genus Brettanomyces. The objects of this study were yeast cultures of Saccharomyces cerevisiae and Brettanomyces bruxellensis. In this work, the cultivations of the yeasts were carried out to compare them as possible sources of beta-glucans. The yeasts were cultivated in a simple periodic culture using a laboratory fermenter (Biostat A, Sartorius). As a result, the content of beta-glucans in the yeasts S. cerevisiae and B. bruxellensis biomass was measured by enzymatic method (Megazyme, Ireland). According to the obtained data, the yeast B. bruxellensis contains a higher amount of beta-glucans than the yeast S. cerevisiae.
Highlights
Any brewery inevitably faces the problem of waste disposal
Residual yeast biomass is often considered as a source of proteins, amino acids, minerals, vitamin B complex, lipids, enzymes and RNA [1, 2]
It is known that one of the types yeast polysaccharides, beta-glucans, in the yeast cell performs the function of structural polysaccharide, is localized in the cell wall and firmly associated with the mannoprotein complex [3,4,5,6,7]
Summary
Any brewery inevitably faces the problem of waste disposal. There is a rising manufacturers interest in recycling by-products. This is important from an economic and from an environmental point of view. Residual brewing yeast – one of the main solid wastes in brewing. Residual yeast biomass is often considered as a source of proteins, amino acids, minerals, vitamin B complex, lipids, enzymes and RNA [1, 2]. Residual brewer's yeasts contain significant amount of carbohydrates (35 to 45% dry biomass). It is known that one of the types yeast polysaccharides, beta-glucans, in the yeast cell performs the function of structural polysaccharide, is localized in the cell wall and firmly associated with the mannoprotein complex [3,4,5,6,7]
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