Abstract

Contamination of brewer's pitching yeast cultures with wild-type yeasts or bacteria is unwanted as it can corrupt the fermentation outcome and causes huge economic losses for the brewing industry. The applicability of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) as a fast tool to monitor the purity of brewer's yeast cultures was investigated. This proof of concept was examined for a brewer's yeast strain contaminated with wild-type yeast and for bottled beer produced by fermentation with that particular contaminated brewer's yeast strain. The data demonstrated that MALDI-TOF MS is very suitable to discriminate between brewing and non-brewing yeast strains. Copyright © 2014 The Institute of Brewing & Distilling

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