Regulation of Estrous cycle using Combination of Gymnema sylvestre and Pergularia daemia in Estradiol Valerate induced PCOS rats

Abstract

Polycystic Ovarian Syndrome (PCOS) is a complex endocrinological and metabolic disorder associated with menstrual dysfunction. In this study, the herbal plant Gymnema sylvestre and Pergularia daemia is used treat the menstrual irregularity by using female albino wistar rat as a model. The experimental animals were divided into five groups each group consisting of six rats. Group I received normal control, Group II received estrodiol valerate 4mg/kg dissolved in 0.4ml sesame oil i.p. on first day considered as a PCOS control, Group III received a Gymnema sylvestre (400mg/kg) for 14 days, Group IV received a Pergularia daemia (200mg/kg) for 14 days, and Group V received a combination of Gymnema sylvestre (400mg/kg) and Pergularia daemia (200mg/kg) for 14 days. The vaginal smear was collected every day to find changes in estrous cycle. The control group showed no changes in the estrous cycle when the EV induced PCOS group had an altered estrous cycle. The Gymnema sylvestre, Pergularia daemia and Gymnema sylvestre+Pergularia daemia treated groups showed better improvement when compared to individual treatment combination treated group potentiality to regain the estrous cycle in the PCOS induced rats.