Abstract

ABSTRACTMagnetospirillum gryphiswaldense is a key organism for understanding magnetosome formation and magnetotaxis. As earlier studies suggested a high genomic plasticity, we (re)sequenced the type strain MSR-1 and the laboratory strain R3/S1. Both sequences differ by only 11 point mutations, but organization of the magnetosome island deviates from that of previous genome sequences.

Highlights

  • Magnetospirillum gryphiswaldense is a key organism for understanding magnetosome formation and magnetotaxis

  • The 550-bp paired-end short-read libraries were prepared with the TruSeq DNA PCR-free LT library preparation kit (Illumina, San Diego, CA), according to instructions given in the TruSeq DNA PCR-free library prep reference guide’s low sample (LS) protocol

  • The libraries were sequenced on an Illumina MiSeq system, employing a MiSeq reagent kit v3 (Illumina) and 600 cycles

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Summary

Introduction

Magnetospirillum gryphiswaldense is a key organism for understanding magnetosome formation and magnetotaxis. The 550-bp paired-end short-read libraries were prepared with the TruSeq DNA PCR-free LT library preparation kit (Illumina, San Diego, CA), according to instructions given in the TruSeq DNA PCR-free library prep reference guide’s low sample (LS) protocol. The libraries were sequenced on an Illumina MiSeq system, employing a MiSeq reagent kit v3 (Illumina) and 600 cycles. Long-read genome assembly was performed using the RS_HGAP_Assembly.3 protocol SMRT Portal version 2.3.0 with standard parameters.

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