Abstract
Reticulocyte ribosomes exposed to 0.5 M KCl are dissociated into subparticles, but they can reconstitute polysomes in the presence of Mg 2+. If these subparticles are isolated by centrifugation they reassociate only in the presence of the KCl-supernatant fluid and Mg 2+. Ribosomes dissociated in KCl are able to support hemoglobin synthesis as components of a complete cell-free system, but subparticles isolated by centrifugation are inactive unless the KCl-supernant fluid is also added. A concentrated KCl-supernatant is able to stimulate hemoglobin synthesis strongly in a reticulocyte cell-free system with intact ribosomes, especially if the ribosomes are preincubated.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
