Rapid Selective TLC-Densitometry Method for Simultaneous Determination of Amoxicillin and Flucloxacillin in their Pure Forms or in their Pharmaceutical Preparation

Abstract

A new sensitive, selective and accurate thin-layer chromatography (TLC) - densitometry method developed for simultaneous estimation of Amoxicillin (AMX) and Flucloxacillin (FLX) in their pure forms or their pharmaceutical formulations. Chromatographic separation was completed on aluminum plate pre-coated with silica gel 60 F254 plates using a mobile phase consist of chloroform: methanol: ammonia (5: 2: 0.5 by volume) on silica gel, followed by densitometry detection of spots at 254 nm . Calibration curves were developed in the range of 4-40 μg/spot and 5-30 μg/spot with good correlation for AMX and FLX, respectively. The adopted method was validated according to ICH guidelines and showed good accuracy and precision. The acquired results were statistically compared with the reported method, where no significant difference has been showed. The developed method can be applied for routine analysis of AMX and FLX in their pure forms and capsule dosage form.