Validated spectrophotometric approach for determination of salbutamol sulfate in pure and pharmaceutical dosage forms using oxidative coupling reaction

Abstract

This study was aimed to find out a simple, sensitive and rapid spectrophotometric method for determination of salbutamol sulfate (SAL). The suggested method was based on the oxidative-coupling reaction of SAL with potassium iodate coupled with 4-amino-5-isopropyl-1-methyl-2-phenyl-1,2-dihydro-3H-pyrazol-3-one (AMP). The formed red colour of the reaction product was measured at a maximum absorption wavelength of 499 nm against the blank solution. It was obeyed to Beer-Lambert's law over the concentration ranged of 2–32 μg mL−1 with a molar absorptivity of 9.165 × 103 L mol−1 cm−1. The suitability of the proposed method for determination of SAL in the presence of some interfering species was studied. The method revealed high sensitivity for determination of the selected drug. The percentage recoveries were in the range from98.8 to 100.4% with relative standard deviation less than 0.3%. The developed method was validated according the current ICH guidelines (ICH guidelines, 2005). Promising results using the proposed spectrophotometric method were recorded for determination of SAL in its pure form and pharmaceutical formulations.