Visible Quantitative Methods for the Determination of Furosemide in Pure form and Pharmaceutical Formulations: A Recent Study

Abstract

The aim of this study is to Design of technical methods for the determination of Furosemide in its pure and pharmaceutical dosage form using spectral methods. This study was planned and executed to estimate Furosemide by using Visible spectrophotometric in pure and pharmaceutical dosage form. This study was carried out at Laboratory of Analytical Research, chemistry department, college of Science, University of Mosul, Mosul-Iraq, during the period of April 2021 to August 2021. Furosemide is the generic name for the medicine Lazix, which is used to treat heart disease and excessive blood pressure. The researchers employed a JASCO V – 630 double-beam computerised UV-Visible spectrophotometer with a 1 cm matched cell and a HANA pH metre to record the pH data. According to findings, Furosemide reacts with bromo-phenol blue, xylenol orange, and chromazorol S. Bromo-phenol blue, xylenol orange were used to measure the decreasing intensity of the resultant coloured complex, while technique (C) was used to measure the growing colour intensity. These three methods were based on charge transfer reaction. The limits of Beer's law for method (A) 0.4-32 \(\mu\)g .mL-1, method (B) 1-32 µg. mL-1, and method (C) were 0.8-32 \(\mu\)g. mL-1 depending on the level of concentration , while the values of the molar absorption coefficient .4×104, 2.1×104 and 1.57×104 l.mol-1.cm-1 for the first, second and third method respectively. Sandel's significance also was calculated for these three methods, 0.0157 \(\mu\)g cm-2 for the first method, 0.0236 \(\mu\)g cm-2 for the second method, while the third method was 0.0210 \(\mu\)g cm-2 . The approach has been used to determine furosemide in both its pure form and in certain of its pharmaceutical formulations with great effectiveness. In terms of linearity, range, accuracy, precision, specificity, and robustness, the proposed approaches were validated. The proposed approaches were effectively used to the estimation of Furosemide in pharmaceutical dosage form, with method (B) being experimentally determined to be the best method based on the best values of molar absorptivity, stability of the resulting complex, and method (B).