Abstract
Spectrophotometric methods were introduced for the simultaneous determination of Amoxicillin (AMX) and flucloxacillin(FLX) in their pure forms and capsules pharmaceutical formulations. The first method was ratio difference (RD), in which the amplitude difference between 238 and 255 nm for AMX and between 254 and 238 nm for FLX were measured.The second one was mean centering of ratio spectra (MCR) in which the peak amplitude at 286nm for the determination of AMX and the peak amplitude at 236 nm for the estimation of FLX were measured. The third method was Area under the curve (AUC) where the range of 226 and 236 nm were adopted for AMX determination and of 240 and 247 nm for FLX determination. All of the previous methods were successfully used for quantitation of AMX and FLX in concentration ranges of 5-50µg/ml and 10-70µg/ml, respectively by RD and MCR methods and of 10-60µg/ml and 10-70µg/ml, respectively for AMX and FLX by AUC method. All of the developed methods were validated using ICH guidelines and statistically compared to a reported method. The adopted methods can be applied for the regular analysis of AMX and FLX mixture in QC laboratories.
Highlights
Amoxicillin (AMX), (6R)-6-[α-D-(4-hydroxyphenyl) glycylamino] penicillanic acid Fig. 1(a) [M.J. O'Neil, 2006], consider one of the most important frequently used βlactam antibiotics in the world
2002.)for the treatment of infections due to staphylococci resistant to treatment by benzyl penicillin .Few methods were reported for determination of both components including spectrophotometry(Attia et al, 2016a; Mohamed, Salem et al, 2007), HPLC (Aly et al, 2007; Liu et al, 2005; Nikam et al, 2009; Shanmugasundaram et al, 2009)], and chemommetric assisted techniques (Attia et al, 2016b; Mohamed et al, 2007)
The best results in according to accuracy and precision showed that the divisor of choice was 50μg/mL of AMX and 50μg/mL of FLX
Summary
Amoxicillin (AMX), (6R)-6-[α-D-(4-hydroxyphenyl) glycylamino] penicillanic acid Fig. 1(a) [M.J. O'Neil, 2006], consider one of the most important frequently used βlactam antibiotics in the world. Amoxicillin (AMX), (6R)-6-[α-D-(4-hydroxyphenyl) glycylamino] penicillanic acid Fig. 1(a) [M.J. Sweetman, 2002.).Flucloxacillin (FLX), (6R)-6-[3-(2chloro-6-fluorophenyl)-5-methylisoxazole-carboxamido] penicillanic acid Fig. 1(b) [M.J. O'Neil, 2006] It is a bactericidal agent used mostly O'Neil, 2006; S.C. The presented methods have the advantage of being easier, economic, rapid and selective than other reported methods . they have the ability to separate Amoxicillin and Flucloxacillin without interference from each other or from capsule excipients
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