Quantitative assessment of the expression of vascular endothelial growth factor and neoangiogenesis in experimental spinal cord ischemia under conditions of combined use of erythropoietin and laser radiation

Abstract

The aim of the work — is to carry out a morphometric assessment of the expression of vascular endothelial growth factor (VEGF) and neoangiogenesis and to justify the combined use of erythropoietin (EPO) and laser radiation (LI) in experimental spinal cord ischemia (EISM) from a pathogenetic standpoint. Materials and methods of research. The work was performed on 300 white nonlinear rats. EISM in rats was created according to the method proposed by G. Z. Sufianova, by total intravasal occlusion of the abdominal aorta and its branches. Histological, immunohistochemical, morphometric, and statistical methods were used in the work. The results of the study. The results obtained allow us to speak about the synergism of the neuroprotective effect of the systemic use of EPO and the local effect of LI in EISM in relation to the studied morphometric parameters in the focus of ischemic damage, including a decrease in the area of edema, the quantitative composition of small blood vessels, VEGF expression, depending on the timing of the experiments. Conclusions. 1. With EISM on days 3, 7, 14, 30 in the brain tissue of small blood vessels, VEGF expression in endotheliocytes is observed, tissue edema is observed. 2. The use of erythropoietin in a total dose of 15,000 IU/kg in EISM leads to an increase in the number of blood vessels in the spinal cord, a decrease in the area of edema. 3. Local single exposure to LI in EISM leads to an increase in the number of small blood vessels in the ischemic area, a progressive increase in the expression of VEGF in endotheliocytes, and a decrease in the area of edema. 4. The combined use of EPO and LI in EISM leads to an increase in the expression of VEGF in endotheliocytes, the number of small blood vessels, and a decrease in the area of edema on days 3, 7, 14 and 30.