Morphometric aspaki the expression of vascular endothelial growth factor in blood vessels and angiogenesis in experimental cerebral ischemia in conditions of combined use of erythropoietin and laser radiation

Abstract

The aim of the work — is to investigate the effect of the combined use of erythropoietin (EPO) and laser radiation (LR) on the morphometric features of the expression of vascular endothelial growth factor (VEGF) and neoangiogenesis in experimental cerebral cortex ischemia (ICGM). Materials and methods of research. The work was performed on 120 white nonlinear rats. ICGM was created by diathermocoagulation of pial vessels in the sensorimotor zone. EPO was applied intraperitoneally at a total dose of 15,000 IU/kg, laser radiation was applied once to the area of the ischemic focus (970 nm, 2 min). The study used histological (staining of sections with hematoxylin and eosin and according to the method of M. Bilshovsky), immunohistochemical (determining the expression of VEGF), morphometric (the number of cells with VEGF expression, the number of small blood vessels were counted, the infarction area was measured), statistical methods of research. The results of the study. The obtained results indicate that the mechanism of the neuroprotective action of EPO is an improvement in microcirculation due to an increase in the number of small blood vessels, and the effect of LR leads to an increase in both the number of blood vessels and an increase in the expression of VEGF in the lesion. Conclusions. 1. During experimental ICGM in rats, the expression of VEGF in the lesion increases and the number of small blood vessels decreases on the 3rd, 7th, 14th, and 30th days of the experiment. 2. Systemic use of EPO in an experimental ICGM at a total dose of 15,000 IU/kg leads to an increase in the number of small blood vessels, and local exposure to the area of LR ischemia leads to an increase in the expression of VEGF and the number of small blood vessels on the 3rd, 7th, 14th, 30th day of the experiment; the representation of blood vessels is higher after exposure to LR compared with the use of EPO. 3. The maximum expression of VEGF and the number of small blood vessels in experimental ICGM were detected after the combined use of EPO and LR.