Abstract
Cardiovascular diseases are the major causes of morbidity and mortality in western countries and the molecular mechanisms responsible are greatly unknown unless a genetic defect or genes/proteins alteration. The biological complexity has shown that one gene did not encode for one protein but for several, due to mRNA splicing and post-translational modifications. Proteomic analysis is a technology that can provide a global approach to study protein modulation and study by differential comparison various samples. Surface-enhanced laser desorption ionization–time of flight (SELDI–TOF) analysis is a technique, based on combination of chromatography of proteins on proteinchip arrays and mass spectrometry, allowing the comparison of protein profiles from various biological samples. This technology has been particularly used for plasma/serum analysis. We will take, as example, the comparative proteomic analysis of plasma from patients having a myocardial infarction by the SELDI approach in describing the principle, the problems linked to the study of plasma, the protein identification and the validation of the technology using other techniques. These approaches have been proved to be interesting for studying cardiovascular diseases in order to better understand the molecular mechanisms involved and identifying protein interactions and new biological factors/biomarkers involved in cardiovascular.
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