Abstract
The article looks into the main problems of determination of iodine trace amounts in multivitamins by inductively coupled plasma-atomic emission spectrometry (ICP-AES), i.e. spectral overlaps attributable to macro-components, and the background effect of organic compounds. The article suggests potential ways of solving these problems. It was shown that there was a minimal overlap of iodine spectral lines by emission lines of multivitamins macro-components at the non-priority wavelength of 182.276 nm. The iodine detection limit (LOD) and limit of quantification (LOQ) were experimentally determined for ICP-AES at different wavelengths. It was demonstrated that LOD and LOQ of iodide ion were much higher in solutions containing a complex organic and multi-element matrix then in water solutions, which calls for the use of spiking in iodine assays. A comparative analysis was performed in which the content of iodine was determined in the multivitamin product Multi-tabs Junior by ICP-AES and by the reference method of inductively coupled plasma-mass spectrometry (ICP-MS). It was demonstrated that the increase in plasma power to 1500 W, the use of spiking which minimizes the background interference of organic compounds, and the use of non-priority wavelength (182.276 nm) make it possible to obtain results of iodine determination in mulitivitamins by ICP-AES which are comparable to the results obtained by the reference method.
Highlights
The article looks into the main problems of determination
of iodine trace amounts in multivitamins by inductively coupled plasma-atomic emission spectrometry
that there was a minimal overlap of iodine spectral lines by emission lines
Summary
В качестве объектов исследования использовали образцы поливитаминного препарата Мульти-табс Юниор с номинальным содержанием йода 135,0– 217,5 мкг/табл. Измерение содержания йода проводили путем сравнения эмиссии испытуемых растворов с эмиссией стандартных растворов иодид-иона с концентрациями 10, 25, 50 мкг/л методом прямой калибровки. Стандартные растворы готовили следующим образом: 0,1 мл раствора стандартного образца иодида аммония с концентрацией иодид-иона 1000 мг/л помещали в мерную колбу вместимостью 100 мл, доводили объем раствора до метки водой деионизированной, тщательно перемешивали. Раствор нагревали на ультразвуковой бане при 70 °С, затем фильтровали и переносили в мерную колбу вместимостью 100 мл, доводя объем раствора до метки 0,5 % раствором гидроксида аммония. Используемых в методе прямой калибровки, в колбы вместимостью 100 мл добавляли соответственно 0,10; 0,25; 0,5; 0,75 и 1,0 мл стандартного раствора иодида калия с концентрацией 1000 мг/л, доводили объем раствора водой деионизированной до метки, тщательно перемешивали. При использовании метода добавок фильтрат делили на три части по 25 мл, одну часть использовали в качестве испытуемого раствора, в другие части добавляли 0,175 и 0,35 мл соответственно стандартного раствора иодида калия с концентрацией 1000 мг/л
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