Abstract
Many bacteria produce extracellular hydrolytic enzymes. These enzymes hydrolyze complex organic compounds into smaller units in the presence of water. Microorganisms can also have endoenzymes functional within cell. Hydrolytic reaction tests play important role in the identification and characterization of microorganisms. Many microorganisms can hydrolyze starch with production of extracellular amylase enzyme. Some microorganisms utilize a particular carbohydrate slowly. Microorganisms utilize different proteins with the production of intracellular and extracellular proteinases and peptidases. Hydrolysis of protein by extracellular enzymes result with formation of amino acids. Following hydrolytic reactions are performed in this practice: protein degradations, urease hydrolysis, litmus milk reactions, starch hydrolysis, casein hydrolysis, and tryptophan hydrolysis.
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