Abstract
The present work has been undertaken for remediating phthalate exposure in the environment. The microbial strain was isolated by enrichment culture technique from the rubbish dump space close to Patna that was contaminated with phthalates for higher degradation ability. The isolated microbial strain T7 was designated as Bacillus cereus after Gram-staining, biochemical characterization, 16S-rRNA sequence and phylogenetic studies. The isolate had the power to utilize 25O μg/ml Di (2-ethyl Hexyl Phthalate) (DEHP) dose taken from 10 mg/ml (DEHP) stock solution within the growth medium. The optimum pH and temperature for DEHP degradation were 8.5 at 37 C. The isolated bacterial strain T7 may allow up to 10% NaCl in minimal salt medium that was enrich with DEHP. The metabolic end product obtained after LCMS was bis [3-(oxolan-2-yl) propyl] nonanedioate having chemical formula C 23 H 40 O 6 . This work provides some new proof for soil rectification by Bacillus species.
Highlights
Phthalates are a class of compounds which is made up of esters of phthalic anhydride, used as plasticizers
Bacterial biomass increased over the whole period of incubation as DEHP was depleted
In the present research work, a microbial strain T7 was efficient to consume DEHP solely as a derivative of carbon and energy was isolated from soil sample which was taken from the rubbish dump space and was identified as B. cereus based on its morphology, gram staining method, different biochemical estimations, and 16S rRNA sequence analysis. 16S rRNA gene sequence analysis of strain T7 showed homology (96%) with that of B. cereus
Summary
Phthalates are a class of compounds which is made up of esters of phthalic anhydride, used as plasticizers. DEHP is amongst the most commonly used plasticizers which are used in many fields such as in industrial, medical and domestic applications. Polyvinyl Chloride (PVC) contains DEHP and when it is disposed of in large excess its leaching in the environment occurs[2]. DEHP affects humans by the aid of damage of liver, developmental and reproductive effects[3,4,5,6]. They have become omnipresent and may be found in the atmosphere, activated sludge, soil, solid waste compost and river, marine and in drinking water[7]. Biological or physicochemical degradation is a way to degrade DEHP from the contaminated environment[9]. Microorganisms could degrade DEHP solely which is a derivative of carbon and energy[10,11,12]
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