Abstract
Rhodella cells grown without added sulphate (-S) divide at a slower rate and secrete sulphated polysaccharide into the medium at a slower rate than control cells. Light and electron microscopy of 20 day -S grown cells show the presence of large numbers of membrane-bound cytoplasmic inclusions with the staining characteristics of polyphosphates (PP). Chemical extraction reveals increases in low and high mol. wt PP in the -S grown cells. The high molecular weight PP has been extracted by the phenol detergent technique and separated from nucleic acids by polyacrylamide gel electrophoresis.
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