Abstract
Indicators of nitrogen limitation were compared in cultured, ex-symbiotic, maltose-releasing Chlorella and in Chlorella freshly isolated from symbiosis with green hydra. Culture of Chlorella at low pH (which stimulates maltose release) caused a reduction in amino acid pool size but no change in the nitrogen to carbon ratio (N: C). In nitrogen-starved cells, both amino acid pool size and N: C decreased. Chlorophyll and protein content, and glutamine to glutamate ratios, indicated that freshly isolated symbiotic Chlorella were more similar to symbionts cultured at low pH than those cultured without nitrogen, while N: C values were intermediate
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