Abstract
The biochemical and biophysical properties of the DNA of herpes simplex virus type 1 (herpes virus-1) and type 2 (herpes virus-2), infectious bovine rhinotracheitis and pseudorabies viruses were compared. Buoyant densities in CsCl were 1.725, 1.727, 1.730 and 1.731 g/cm 3 for the DNAs of herpes virus-1, -2, infectious bovine rhinotracheitis virus and pseudorabies virus, respectively. Base composition analysis was conducted by enzymatically hydrolyzing viral DNA and calculating the nucleotide composition from elution patterns obtained from anion-exchange column. From these patterns, the guanine plus cytosine content was calculated to be 69.9% for herpes virus-1 DNA, 72.0% for herpes virus-2 DNA, and 76.7% for pseudorabies virus DNA. When thermally denatured in 0.015 M NaCl-0.0015 M sodium citrate, the DNAs of herpes virus-1, -2, infectious bovine rhinotracheitis virus and pseudorabies virus yielded average T m values of 82.6°, 82.7°, 85.6° and 86.6°, respectively. The DNAs of herpes virus-1 and -2 had the same sedimentation coefficient (56 S) in neutral Tris-saline buffer, corresponding to a molecular weight of 88·10 6. Pseudorabies virus DNA sedimented as two components with sedimentation coefficient values of 26 S and 31 S.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
