Permanent Draft Genome Sequence of Desulfurococcus amylolyticus Strain Z-533T, a Peptide and Starch Degrader Isolated from Thermal Springs in the Kamchatka Peninsula and Kunashir Island, Russia.

Dwi Susanti,Alla Lapidus,James Han,Eric F Johnson,Nikos C Kyrpides,Biswarup Mukhopadhyay,Manoj Pillay,T B K Reddy,Anna A Perevalova,Tanja Woyke,Natalia N Ivanova,Supratim Mukherjee

doi:10.1128/genomea.00078-17

Abstract

ABSTRACTDesulfurococcus amylolyticus Z-533T, a hyperthermophilic crenarcheon, ferments peptide and starch, generating acetate, isobutyrate, isovalerate, CO2, and hydrogen. Unlike D. amylolyticus Z-1312, it cannot use cellulose and is inhibited by hydrogen. The reported draft genome sequence of D. amylolyticus Z-533T will help to understand the molecular basis for these differences.

Highlights

Desulfurococcus amylolyticus Z-533T, a hyperthermophilic crenarcheon, ferments peptide and starch, generating acetate, isobutyrate, isovalerate, CO2, and hydrogen
The 16S rRNA gene sequences of D. amylolyticus Z-533T differ by 0.1 to 0.3% from those of other D. amylolyticus strains, namely, Z-1312 and 1221n [2,3,4], which were formerly known as D. fermentans Z-1312 and D. kamchatkensis 1221n, respectively [5]
A genomic analysis of three desulfurococci will give insight into the mechanisms by which D. amylolyticus Z-1312 degrades cellulose and by which other strains are inhibited by hydrogen

Summary

Introduction

Desulfurococcus amylolyticus Z-533T, a hyperthermophilic crenarcheon, ferments peptide and starch, generating acetate, isobutyrate, isovalerate, CO2, and hydrogen. The 16S rRNA gene sequences of D. amylolyticus Z-533T differ by 0.1 to 0.3% from those of other D. amylolyticus strains, namely, Z-1312 and 1221n [2,3,4], which were formerly known as D. fermentans Z-1312 and D. kamchatkensis 1221n, respectively [5]. Only D. amylolyticus Z-1312 degrades cellulose [2] and is not inhibited by the presence of hydrogen [6].

Results

Conclusion