Patients with visceral leishmaniasis present an increased expression of TLR2 and TLR4 in lymphocytes and lipid and protein changes

Abstract

Event Abstract Back to Event Patients with visceral leishmaniasis present an increased expression of TLR2 and TLR4 in lymphocytes and lipid and protein changes Mariana Gatto1, Mariana M. Abreu1, Marjorie D. Golim2, José C. Simão3, Karen I. Tasca1*, Loraine Gollino1, Carlos M. Fortaleza1, Paulo C. Pereira1 and Sueli A. Calvi1 1 Univ Estadual Paulista - UNESP, Tropical Disease, Brazil 2 Univ Estadual Paulista - UNESP, Hemocenter Laboratory of Flow Citometry, Brazil 3 Manoel de Abreu Hospital, Brazil In order to have the development of adaptive immune response in visceral leishmaniasis is necessary that cellular receptors such as TLR2 and TLR4 recognize and play the parasite effector mechanisms. Moreover, the nutritional profile also has impact on immune response. Our objective was to evaluate the expression of TLR2 and TLR4 in CD14 and CD3 cells and correlate with nutritional status in patients with visceral leishmaniasis, pre and post-treatment. Were evaluated 8 patients pre-treatment, 3 post-treatment and 8 healthy subjects. Analysis of the expression of TLR2 and TLR4 receptors on CD3 and CD14 cells was performed by flow cytometry and the nutritional profile was performed by bioelectrical impedance and biochemical tests. In CD3 cells, expression of TLR2 was higher in patients pre-treatment than those post-treatment (p <0.05) and controls (p <0.05); TLR4 expression was higher in pre-treatment compared to controls (p <0.05). In CD14 cells, TLR2 expression was lower in pre-treatment compared to controls (p <0.05) and TLR4 expression did not differ between groups. Levels of HDL and albumin were lower in pre-treatment compared to post-treatment (p <0.05) and controls (p <0.05), phase angle was lower in pre-treatment than those post-treatment (p <0.05) and triglycerides were higher in pre-treatment compared to controls (p <0.05). Our results suggest that patients with LV activates expressed primarily TLR2 and TLR4 in lymphocytes, suggesting a possible involvement of these receptors in the development of the immune response. Patients also showed nutritional changes that could be interfering with the immune response against the parasite. Keywords: Visceral leishmaniasis, Innate response, TLRs (Toll-like receptors), Nutritional Status, pre and pós-treatment Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Host-pathogen interactions Citation: Gatto M, Abreu MM, Golim MD, Simão JC, Tasca KI, Gollino L, Fortaleza CM, Pereira PC and Calvi SA (2013). Patients with visceral leishmaniasis present an increased expression of TLR2 and TLR4 in lymphocytes and lipid and protein changes. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00517 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 02 May 2013; Published Online: 22 Aug 2013. * Correspondence: Miss. Karen I Tasca, Univ Estadual Paulista - UNESP, Tropical Disease, Botucatu, SP, Brazil, karenitasca@hotmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Mariana Gatto Mariana M Abreu Marjorie D Golim José C Simão Karen I Tasca Loraine Gollino Carlos M Fortaleza Paulo C Pereira Sueli A Calvi Google Mariana Gatto Mariana M Abreu Marjorie D Golim José C Simão Karen I Tasca Loraine Gollino Carlos M Fortaleza Paulo C Pereira Sueli A Calvi Google Scholar Mariana Gatto Mariana M Abreu Marjorie D Golim José C Simão Karen I Tasca Loraine Gollino Carlos M Fortaleza Paulo C Pereira Sueli A Calvi PubMed Mariana Gatto Mariana M Abreu Marjorie D Golim José C Simão Karen I Tasca Loraine Gollino Carlos M Fortaleza Paulo C Pereira Sueli A Calvi Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.