Can cytokine profiles in HIV patients be useful in clinical practice?

Abstract

Event Abstract Back to Event Can cytokine profiles in HIV patients be useful in clinical practice? Karen I. Tasca1*, Mariana Gatto1, Caio Cassavan De Camargo1, Sueli A. Calvi1, Alexandrina Sartori1 and Lenice R. Souza1 1 Univ Estadual Paulista - UNESP, Tropical Disease, Brazil HIV infection causes an accentuated dysregulation on cytokine production. These changes have been extensively studied in patients on antiretroviral therapy (ART). The aim of this study was to characterize the cytokine profile in 58 asymptomatic HIV-patients, according to different answer to ART. The following groups were enrolled: naïve to ART (HIVn, n=18); on ART but presenting incomplete viral suppression (HIVi, n=18); on ART and presenting complete viral suppression (HIVc, n=22). Quantification of viral load and CD4+ T cells were done by branched-DNA and flow cytometry, respectively. Sandwich immunosorbent assays were performed to quantify cytokines as IFN-g (Th1 profile), TNF-a (Th1 and Th17), IL-6, IL-17 (Th17) and IL-10 (Th2 and Treg). The asymmetric variables were analyzed by negative binomial and gamma distributions (p<0.05); normal variables were analyzed by ANOVA followed by Tukey (p<0.05). The HIVc group presented the lowest concentrations of IL-10 and IL-17. The HIVi group presented intermediate levels of IL-17, the lowest levels of IFN-g and the highest of IL-6 and TNF-a; a positive correlation between these last two cytokines was observed. HIVn showed the highest level of IL-17. This cytokine was the only one that differentiated the 3 groups (HIVn > HIVi > HIVc). These findings suggest the cytokine profile can be useful in clinical practice to characterize the immunological status of asymptomatic patients. Keywords: HIV, aids, Cytokines, antiretroviral therapy, T cells profile Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Host-pathogen interactions Citation: Tasca KI, Gatto M, Cassavan De Camargo C, Calvi SA, Sartori A and Souza LR (2013). Can cytokine profiles in HIV patients be useful in clinical practice?. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00551 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 01 May 2013; Published Online: 22 Aug 2013. * Correspondence: Miss. Karen I Tasca, Univ Estadual Paulista - UNESP, Tropical Disease, Botucatu, SP, Brazil, karenitasca@hotmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Karen I Tasca Mariana Gatto Caio Cassavan De Camargo Sueli A Calvi Alexandrina Sartori Lenice R Souza Google Karen I Tasca Mariana Gatto Caio Cassavan De Camargo Sueli A Calvi Alexandrina Sartori Lenice R Souza Google Scholar Karen I Tasca Mariana Gatto Caio Cassavan De Camargo Sueli A Calvi Alexandrina Sartori Lenice R Souza PubMed Karen I Tasca Mariana Gatto Caio Cassavan De Camargo Sueli A Calvi Alexandrina Sartori Lenice R Souza Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.