Abstract

Paenibacillus larvae bacteriophage Tripp was isolated from an American foulbrood diseased honey bee hive in North Carolina, USA. The 54,439-bp genome is 48.3% G+C, encodes 92 proteins, no tRNAs, and has 378-bp direct terminal repeats. It is currently unique in Genbank.

Highlights

  • Paenibacillus larvae bacteriophage Tripp was isolated from an American foulbrood diseased honey bee hive in North Carolina, USA

  • Bacteriophage Tripp was isolated on Paenibacillus larvae strain ATCC 9545, a bacterium that causes American foulbrood disease (AFB) of honey bees [1]

  • Comb swab samples from the frame of an AFB diseased hive in Lincoln County, North Carolina, USA were incubated in brain heart infusion broth plus thiamine and glucose with P. larvae 9545

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Summary

Introduction

Paenibacillus larvae bacteriophage Tripp was isolated from an American foulbrood diseased honey bee hive in North Carolina, USA. Bacteriophage Tripp was isolated on Paenibacillus larvae strain ATCC 9545, a bacterium that causes American foulbrood disease (AFB) of honey bees [1]. A phage Tripp plaque was purified three times and amplified using the whole plate lysis method.

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