Optimization and Production of Hyaluronidase by Streptococcus mitis MTCC 2695

Narayanan Mahesh

doi:10.4172/2167-7956.1000101

Abstract

Hyaluronidase was produced by submerged fermentation from Streptococcus mitis. The possibility of using Streptococcus mitis for enzyme production has been recently investigated. In this study, the physical and nutritional parameters were optimized to improve the production of hyaluronidase by Streptococcus mitis and it was accessed. Maximum production of hyaluronidase was obtained when 5% starch supplemented as carbon source achieved by employing (98.7U/ml) and followed by ammonium chloride (140.4U/ml) incubation period about 48 hours showed (108.9U/ml) and temperature at 37°C showed (179.9U/ml). The maximum enzyme yield on pH 4 is (110.7U/ml). The production of hyaluronidase by means of immobilized Streptococcus mitis was evaluated and a maximum production was obtained with the medium was inoculated with 100 beads (591U/ml) which was more than that of mobilized cells.

Highlights

Hyaluronidase is an enzyme which hydrolyses hyaluronic acid, a high molecular weight non-sulfated linear glycosaminoglycan, which is composed of repeating disaccharide units, D-glucuronic acid and Nacetyl glucosamine [1]
Effect of various carbon sources on hyaluronidase production Various carbon sources were added to the fermentation medium for the production of hyaluronidase at 0.05% concentration
The present research showed that higher levels of hyaluronidase produced at increasing agitation values and the time of fermentation with the maximum values are produced with its satisfactory yields

Summary

Introduction

Hyaluronidase is an enzyme which hydrolyses hyaluronic acid, a high molecular weight non-sulfated linear glycosaminoglycan, which is composed of repeating disaccharide units, D-glucuronic acid and Nacetyl glucosamine [1]. Hyaluronidase can be produced by submerged fermentation from Streptococcus mitis [3,4,5]. It is a valuable enzyme because it can acts as an adjuvant, which accelerates and increases the absorption and dispersion of injected drugs [6]. Widely distributed in nature, hyaluronidases are not well characterized and are a group of neglected enzymes owing to their difficult purification and lack of scientific interest over a large period of time [7]. Hyaluronidase was useful in the direct reduction of hyaluronic acid that was improperly placed during injection [8,9,10]

Methods

Results

Conclusion