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Abstract
1. 1. The effect of erythropoietin on the stimulation of hemoglobin synthesis by marrow cells is counteracted by actinomycin D, if the inhibitor is added before the hormone or shortly after. 2. 2. After 24 h of contact with erythropoietin, marrow cell hemoglobin synthesis is resistant to the effect of actinomycin D. 3. 3. Stimulated hemoglobin synthesis is largely, but not completely inhibited, by colchicine. 4. 4. These findings are discussed with reference to the mechanism of action of erythropoietin and its possible role as a de-repressor in the induction of erythroid differentiation.
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