Nanoscale sulfur alleviates silver nanoparticle toxicity and improves seed and oil yield in Soybean (Glycine max)

Abstract

Silver nanoparticles (AgNPs) are commonly used in many commercial products due to their antimicrobial properties, and their significant exposure in agricultural systems is anticipated. AgNPs accumulation in soil and subsequent uptake by plants can be harmful to plant growth and exposure to animals and humans through the food chain is a major concern. This study evaluated the potential protective role of nanosulfur (NS) and bulk sulfur (BS) at 200 and 400 mg/kg soil application in alleviating silver nanoparticle (AgNPs; 32 and 64 mg/kg) phytotoxicity to soybean [Glycine max (L) Merr.]. The treatments were added in the soil before soybean transplantation; growth, yield, nutrient, and silver accumulation were measured in the shoot, root, and seeds. Exposure to AgNPs significantly affected plant growth and yield, reducing nodule weight by 40%, fresh shoot weight by 66%, and seed yield by 68% when compared to controls. However, nanosulfur application in soil alleviated AgNPs toxicity, and importantly, this impact was nanoscale specific at the higher concentration because the benefits of corresponding bulk sulfur (BS) treatments were marginal. Specifically, nanosulfur at 400 mg/kg significantly increased seed yield (∼3-fold more than AgNP at 64 mg/kg) and shoot biomass (2.6-fold more than AgNP at 64 mg/kg) upon co-exposure with AgNPs, essentially alleviating AgNPs toxicity. Moreover, NS increased nodule mass by 3.5 times compared to AgNPs-treated plants, which was 170% greater than the Ag- and NS-free controls. Plants treated with NS with AgNPs co-exposure accumulated significantly less Ag in the shoots (∼80% reduction) and roots (∼95% reduction); no Ag contents were detected in seeds. These findings demonstrate the potential of sulfur, especially NS, as a sustainable soil amendment to reduce the accumulation and toxicity of AgNPs and as a valuable nano-enabled strategy to promote food safety and security.