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Abstract
1. 1. Disaccharidases from normal human intestinal mucosa have been solubilised by “autolysis” and/or papain (EC 3.4.4.10) digestion and have been partially separated by gel filtration on Sephadex G-200. 2. 2. With both types of solubilization used several maltases (maltose glucohydrolases) were found, some of which also have sucrase (sucrose glucohydrolase) of isomaltase (isomaltose glucohydrolase) and palatinase activities. 3. 3. With the same technique the existence of two lactases (lactose galactohydrolases) was demonstrated. Both of them attack cellobiose also. No other cellobiase (cellobiose glucosehydrolase) was found.
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