Multi-omics joint analysis of the effect of temperature on microbial communities, metabolism, and genetics in full-scale biogas reactors with food waste

Abstract

Due to the diversity of microbiota and the high complexity of their interactions that mediate biogas production, a detailed understanding of the microbiota is essential for the overall stability and performance of the anaerobic digestion (AD) process. This study evaluated the microbial taxonomy, metabolism, function, and genetic differences in 14 full-scale biogas reactors and laboratory reactors operating under various conditions in China. This is the first known study of the microbial ecology of AD at food waste (FW) at a regional scale based on multi-omics (16S rRNA gene amplicon sequencing, metagenomics, and proteomics). Temperature significantly affected the bacterial and archaeal community structure (R2 = 0.996, P = 0.001; R2 = 0.846, P < 0.002) and total inorganic carbon(TIC) slightly changed the microbial structure (R2 = 0.532, P = 0.005; R2 = 0.349, P = 0.016). The Wood-Ljungdahl coupled with hydrogenotrophic methanogenic pathways were dominant in the thermophilic reactors, where the acs, metF, cooA, mer, mch and ftr genes were 10.1-, 2.8-, 16.2-, 1.74-, 4.15-, 1.04-folds of the mesophilic reactors (P < 0.01). However, acetoclastic and methylotrophic methanogenesis was the primary pathway in the mesophilic reactors, where the ackA, pta, cdh and mta genes were 2.2-, 3.2-, 14.3-, 1.88-folds of the thermophilic group (P < 0.01). Finally, the Wilcoxon rank-sum test was applied to explain the cause of the temperature affecting AD microbial activities. The findings have deepened the understanding of the effect of temperature on AD microbial ecosystems and are expected to guide the construction and management of full-scale FW biogas plants.