Abstract
Muscle glycogen appears in the form of single beta-granules which become visible after staining with lead. Treatment of tissue with uranyl acetate before dehydration (en bloc) causes an apparent disappearance of glycogen. However, histochemical studies have shown that at least a part of glycogen remains in the tissue (5). In our work on the conducting tissue in the dog heart various degrees of glycogen disappearance were observed after uranyl acetate treatment of the tissue (3). The present report includes ultrahisto- chemical analysis of those samples that showed a high degree of glycogen disappearance and a tentative explanation of the effects of uranyl acetate on glycogen structure and staining.Dog heart was fixed by perfusioninvivo with 3% glutaraldehyde in 0.1 M phosphate buffer, pH 7.2. Excised conducting fibers were fixed overnight in 6% glutaraldehyde, post fixed in OsO4, for 15 minutes, processed either routinely or with uranyl acetate, and embedded in Spurr's epoxy resin.
Published Version
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