Molecular detection of carbapenemase resistance in Klebsiella pneumoniae isolated from urine of patients assessing General Hospital in Keffi, Nasarawa state, Nigeria

Abstract

Abstract Background: Carbapenem resistance in K. pneumoniae have been reported to emerge in developing and developed countries. Studies on molecular detection of Carbapenem resistance gene in Imipenem resistant K. pneumoniae isolated from urine of patients with suspected Urinary tract infection in General Hospital, Keffi, Nigeria. was carried out. Methods: A total of two hundred and ten (210) urine samples were collected from patients and K. pneumoniae was isolated and identified using standard microbiological method. The Antibiotic susceptibility test for the isolates was carried out and interpreted in accordance with Clinical and Laboratory Standard Institute (CLSI) protocol. The Molecular detection of Carbapenem resistance gene were carried out using Polymerase Chain Reaction (PCR) method. The occurrence of K. pneumoniae was 46(23.3%). Results: The highest occurrence of K. pneumoniae in relation to age was observed at age 21-30 (27.1%) and lowest was at age≥ 50 (15.00%). In relation to gender the occurrence was higher in females (32.6%) than male (15.7%). The antibiotic resistance of K. pneumoniae showed that the isolates were more resistant to Sulphamethoxazole/Trimethoprim (89.7%) and less resistant to Gentamicin (30.6%) and Imipenem (16.3%). The occurrence of Multidrug resistance (MDR) and pan drug resistance (PDR) isolates were in order of occurrence MDR (83.6%) >PDR (16.3) > XDR (0.0%). The percentage occurrence of Carbapenem resistance gene in K. pneumoniae isolates were blaKpc (100%) positive and blaVIM (33.3%) positive. Conclusion: The occurrence of K. pneumoniae isolates from urine of suspected urinary tract infections of patients in this study location was high and antibiotics such as Cefexime, Gentamicin and Imipenem were very effective against the K. pneumoniae isolates. Also, most of the isolates were multi-drug resistance (MDR). In addition, KPC and VIM genes were predominantly detected in imipenem resistant isolates.