MODIFICATION OF THE METHOD OF QUANTITATIVE DETERMINATION OF FLAVONOIDS IN THE GRASS OF THE MOUNTAIN BIRD POLÝGONUM AVICULÁRE L.

Abstract

Аbstract Aim– Modification a methodology of the quantitative content of the sum of flavonoids in the raw material of bird's knotweed (Polýgonum Aviculáre L.) Material and methods. The raw materials of the bird's mountaineer (grass) were collected near the village of Ponomarevka, Dombarovka, Alekseevka, Orenburg region in June-July 2022. The collected material was dried in accordance with the requirements of the GF. The quantitative content was studied using a Vilitek VBS ultrasonic bath at a temperature of 40 ° C and a power of 60 W, a LAB-TB-6/W water bath at a temperature of 90 ° C, a rotary evaporator "IR-1 LT" at a temperature of 70 ° C. The electronic spectra were measured on a UNICO 2800 UV spectrophotometer, in 10 mm thick cuvettes in the wavelength range from 190 to 700 nm. The comparison solution was prepared using 96% ethyl alcohol concentration. Results. The presented method of quantitative determination can be used in "end-to-end" standardization, for raw materials of other types of mountaineer, as well as medicinal herbal preparations based on mountaineers. We selected the extraction conditions using 50% ethyl alcohol in a ratio (raw material-extractant) of 1:50 and boiling in a water bath for 60 minutes, which allowed us to achieve the yield of the amount of flavonoids more than 0.5% declared in GF XIV (FS.2.5.0069.18). Conclusion. New, different from pharmacopoeia conditions for the analysis of the quantitative determination of the amount of flavonoids in the grass of the mountaineer bird are proposed. The main differences, which is a single extraction, in comparison with a three-stage pharmacopoeia; reduction of extraction time in a water bath to 60 minutes, which is 30 minutes less than in the method of GF XIV; the concentration of the extractant and the ratio of "raw materials:extractant". Thus, the method of quantitative determination of the grass of the mountain bird has been modified according to the basic conditions of extraction of raw materials. As a result, based on the data obtained, we can recommend the content of flavonoids in the raw material of bird's knotweed at least 1.5 ± 0.08%.