DEVELOPMENT OF FLAVONOIDS QUANTITATIVE DETERMINATION METHOD IN GLYCYRRHIZA PALLIDIFLORA HERB (GLYCYRRHIZA PALLIDIFLORA MAXIM.)

Abstract

The licorice genus (Glycyrrhiza L.) plants are widely used in conventional and traditional medicine in different countries around the world. The genus includes about 15 species, but only three of them - G. glabra, G. uralensis and G. inflata are well studied and included in the Europe and Asia pharmacopoeias. Pale-flowered licorice (Glycyrrhiza pallidiflora Maxim.) is a perennial herbaceous plant from the false (not sweet) licorice section (Pseudoglycyrrhiza Krug.), which do not accumulate glycyrrhizin derivatives. This East Asian species grows in the Russian Far East. The distribution area of G. pallidiflora is limited. Only a few of its geographically isolated habitats are known: within the Khabarovsk city, near the Sarapulskoe village (Lower Amur) and in the Primorsky Kray on the Ryabokon Peninsula on the Lake Hanka southeastern coast. The G. pallidiflora successful introduction on the Kemerovo region-Kuzbass territory has been carried out over the past 5 years in the “Apothecary garden” territory of the Kuzbass Botanical Garden of the Institute of Human Ecology, FRC UUH SB RAS, Kemerovo. Currently, the scientific literature contains limited information on the composition and quantitative content of biologically active compounds (BAC) in the aerial part of G. pallidiflora. Therefore, the main task is to conduct phytochemical studies of the main classes of BAC, which have a pronounced pharmacological activity. We have proposed the amount of flavonoids quantitative determination method in the pale-flowered licorice herb, introduced in Kuzbass, by the differential spectrophotometry method, based on aluminum (III) chloride complexation reaction. The flavonoids extraction conditions from raw materials have been studied. They depend on the ethyl alcohol concentration, the plant raw materials grinding degree, the extraction time, and the raw materials and extractants ratio. The optimal extraction conditions were selected and a quantitative determination method was developed. On the base of it was established that the amount of flavonoids content in the herb of pale-flowered licorice (Glycyrrhiza pallidiflora Maxim.), in terms of rutin, is from 2.25 ± 0.38% to 2.44 ± 0, 04%. The relative error of the proposed method is ± 3.21%.