Abstract
Rat liver chromatin was fractionated into DNA, histones and non-histone chromosomal proteins and each component was modified with N- methyl-N′- nitro-N- nitrosoguanidine or N- ethyl-N′- nitro-N- nitrosoguanidine . The radioactivity of 14C-labeled alkyl or guanidino moieties of both compounds bound significantly to both histones and non-histone chromosomal proteins and the binding of N- methyl-N′- nitro-N- nitrosoguanidine was higher than N- ethyl-N′- nitro-N- nitrosoguanidine . However the binding of both compounds to DNA was very low and its significance was hard to evaluate. All of the three components, one of which was modified, were reconstituted into chromatin, then, [ 3H]UMP incorporation into acid insoluble material using Escherichia coli RNA polymerase (EC 2.7.7.6) was measured. Only with the reconstituted chromatin containing histones modified either by N- methyl-N′- nitro-N- nitrosoguanidine or N- ethyl-N′- nitro-N- nitrosoguanidine , the template activity increased drastically; i.e., about 10 or 5 times higher than that with the unmodified reconstituted chromatin, respectively. However, any remarkable alteration in the electrophoretic pattern of protein fraction of the reconstituted chromatin could not be found. The results obtained in this study are discussed in the context that the modified histones could give rise to change in the mutual interaction of chromosomal components during the reconstitution of chromatin accompanied with the increase of chromatin template activity.
Published Version
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