Microscope observations and DNA analysis of wine residues from Roman amphorae found in Ukraine and from bottles of recent Tuscan wines

Abstract

Morphological analysis by light and electron microscopy was conducted on wine residues from second century Roman amphorae found in Myrmekion (Ukraine). The results of the archaeological samples were compared with residues formed in bottles of wine from Tuscan vineyards, corked between 1969 and 1977 without filtration and enzyme or biochemical processing. Staining and histochemical observation of the archaeological and recent residues detected nucleic acids. Molecular analysis was also performed using nuclear microsatellite SSRs markers having high polymorphism to study genetic relationships. Genotype profiles of archaeological and recent residues were compared with contemporary cultivars in a data bank. Low homology of genotype profiles of all residues, and oral evidence, confirmed the presence of autochthonous varieties in recent wines and enabled indirect assessment of varieties detected in the archaeological material. The results confirmed that the archaeological material could be related to Sangiovese and indicated Roman wine trade as far afield as Ukraine, whereas the recent residues provided evidence of disappearing native Tuscany cultivars like Gorgottesco, Mammolo, Verdello, Rossone and Tenerone, used for table wines until the late seventies.