Abstract
Ovarian cancer (OC) develops asymptomatically and is not diagnosed until advanced stages, which increases the mortality rate from this disease. In the diagnosis and treatment of OC, new prospects have been opened in studies of the gene regulation mechanisms involving long non-coding RNAs (lncRNAs) and identification of lncRNA genes inhibited by methylation of promoter regions. Using a set of 122 samples of primary OC tumors, by methylation specific real-time PCR, changes in the methylation level of a group of lncRNA genes were studied: PLUT, SNHG1, SNHG6, SNHG12, and TINCR. Using the nonparametric Mann–Whitney test, a statistically significant (p 0.001) increase in the methylation level of these 5 lncRNA genes in tumors was shown. A statistically significant (p 0.05) correlation was established between the level of methylation of the lncRNA genes SNHG6, SNHG12 and TINCR with the stage of the tumor process, the histological grade and the presence of metastases. Using real-time RT-qPCR, a decrease in the expression level of the SNHG6, SNHG12 and TINCR genes was observed and a significant correlation of methylation with the expression of SNHG6 and TINCR was shown (rs ≤ −0.5, p 0.001). Thus, new lncRNA genes representing potential epigenetic markers of ovarian cancer have been identified.
Published Version
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