Abstract
Preeclampsia is a major cause in both maternal and perinatal mortality and morbidity. The etiopathogenesis of preeclampsia is still not fully elucidated but it is believed to be a multifactor. It is assumed that preeclampsia is caused by oxidative stress in placenta which cause endothelial and vascular dysfunction. In vitro model research is considered the best and most effective way to understand the disease pathophysiology. Phaleria macrocarpa (Scheff.) Boerl also known as Mahkota Dewa is widely used as an antioxidant because of alkaloids, saponins, flavonoids and polyphenols properties. The phenol and flavonoid compounds have antioxidant and anti-inflammatory activity. This study aimed to determine Phaleria macrocarpa extract‘s LC50 in fibroblast cell primary culture. The LC50 was measured based on Brine Shrimp Lethality Test (BSLT) method. The LC50 of Ethyl Acetate fraction of Phaleria macrocarpa extract was 38.16 ppm and the LC50 of N-Hexane Fraction of Phaleria macrocarpa extract was 157.91 ppm, both measured at 550 nm wavelength. Phaleria macrocarpa extract‘s LC50 obtained later can be used as reference in subsequent research using trophoblast cell and HUVEC cultures. Further studies regarding the optimal dose of Phaleria macrocarpa extract for clinical trial are encouraged.
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