Abstract
ABSTRACTHuman sapovirus (SaV) is responsible for severe gastroenteritis among infants and children. Research about the genetic configuration of SaV is scarce in Bangladesh. Here, we report the complete genome sequence of an SaV strain with intragenogroup recombination, isolated from an infant with severe diarrhea in Bangladesh in 2005.
Highlights
Human sapovirus (SaV) is responsible for severe gastroenteritis among infants and children
The stool sample collected from the patient was diluted with 10% phosphatebuffered saline (PBS) and centrifuged at 5,000 rpm for 10 min
SaV cDNA was synthesized from RNA using a cDNA kit (Applied Biosystems, USA), and the complete genome was amplified by PCR, using eight sets of primer pairs spanning the entire genome
Summary
Human sapovirus (SaV) is responsible for severe gastroenteritis among infants and children. The stool sample collected from the patient was diluted with 10% phosphatebuffered saline (PBS) and centrifuged at 5,000 rpm for 10 min.
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