Abstract
Organochlorine pesticides (OCPs) have caused increasing global concern due to their high toxicity, persistence, bioaccumulation, and significant adverse effects on human health. This study was to explore the interaction effects between OCPs and isoflavones. Six kinds of OCPs and 2 kinds of isoflavones-genistein and daidzein were included to study their effect on MCF-7 cells in vitro. Eighty-one female Sprague-Dawley rats were randomized to 9 groups according to factorial design to study the interaction effect between isoflavones and γ-HCH. Compared to organochlorine pesticides alone group, proliferation rate of MCF-7 cells was lower in 100 μmol/L genistein + organochlorine pesticides and 100 μmol/L daidzein + organochlorine pesticides group (p < 0.05). In vivo study showed that there are interaction effects on kidney weight and liver weight when treated with isoflavones and γ-HCH. The changes in uterine morphology and positive expression of ERα showed inhibition effects between isoflavones and γ-HCH. In conclusion, the data suggests that there are interactions between isoflavones and OCPs in vitro and in vivo.
Highlights
The research on endocrine-disrupting chemicals (EDCs) has attracted more and more interest of scientists
Organochlorine pesticides (OCPs) could increase proliferation rate of MCF-7 cell line while genistein and daidzein could inhibit the growth of MCF-7 cell observably (p < 0.05)
Compared with OCPs alone group (o,p-DDT, p,p-DDT, β-HCH, and γ-HCH), proliferation rate of MCF-7 cells was lower in both 100 μmol/L genistein + OCPs group and daidzein + OCPs group (p < 0.05)
Summary
The research on endocrine-disrupting chemicals (EDCs) has attracted more and more interest of scientists. Organochlorine pesticides (OCPs), which are one of the EDCs, can be lipophilic, stable, difficult to be degraded, volatile, and accumulative They are easy to be accumulated in environment, and humans can absorb them via food chain enrichment through the digestive tract, respiratory tract, and skin [1, 2]. Exposure of men or animals to OCPs can cause some side effects including reproductive toxicity and even cancer [3, 4]. A large number of studies showed that OCPs could combine with estrogen receptor to cause some side effects including reproductive toxicity [7, 8]. High concentrations of genistein can inhibit the proliferation of cells while DDTs promote the proliferation of cells, the effects of isoflavones on breast cancer remain controversial and human clinical investigations are needed [10]. It has practical significance to carry out research on interactions between OCPs and isoflavones
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