（I）利用高效能液相層析法建立山藥及山楂的化學指紋圖譜（II）應用固相微萃取法配合幾丁聚醣來分析及處理水中的有機氯殺蟲劑

Abstract

Part I In the study, we use the high performance liquid chromatograph combined photodiode array(PDA) UV/Vis detector for analyzing the Chinese herb “Shanyan” and “Beishanzha” which came from different producing areas. The chemical fingerprints of herbs were established and compared from the chromatograms by the wavelength 210nm, 230nm, 254nm, 280nm for detection. According to the chromatograms, we choose the several peaks which are more abundance as the characteristic peaks. Additionally, to compare the isocratic elution and gradient elution two systems, we find that it is obviously better to use the gradient elution. Finally, we use the data from the chromatograms to make simple, easy and clear chemical fingerprint and radar atlas of Chinese herb. This radar atlas of Chinese herb can give the information of their sources and producing areas. Part II In the study, we developed a method for analyzing and treatment of organochlorine pesticides in water by solid-phase microextraction(SPME) combined with chitosan as an adsorbent. Organochlorine pesticides(OCPs) are generally found in water at ppt levels, however, a preconcentration step prior to the chromatographic analysis is necessary. SPME is rapid, simple and solvent-free and sensitive technique. We use the PDMS/DVB fiber and headspace SPME(HS-SPME) method to extract the OCPs in water. After the sampling, we inject the sample to the GC/ECD for analysis. In the study. we analyzed the eighteen OCPs from water samples. Chitosan is a polymer of glucosamine, they have the absorbance characterstics and chitosan also chelate with metal ions, so they can be used as the materials for the pesticides treatments. The water samples after the treatment, we find that nine kinds of OCPs can be effectively treatment to 100％, and the other three kinds are 80~93％.