Abstract
Administration of insulin to alloxan-diabetic rats stimulated equally the incorporation of [ 14C]orotic acid or 32P 1 into liver RNA within 30–60 min after injection. RNA polymerase (nucleosidetriphosphate: RNA nucleotidyltransferase, EC 2.7.7.6) activity of isolated liver nuclei was also stimulated by insulin in vivo. This activity rose measurably within 2 h after insulin injection and reached peak levels (2.3-fold increase) after 36 h of insulin treatment. Addition of insulin to nuclei in vitro did not affect RNA polymerase activity. The rise in RNA polymerase activity after insulin was almost completely blocked by the simultaneous administration of puromycin, suggesting a requirement for protein synthesis in this response.
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