Inhibition of PGE2 and LTB4 production by dendritic cells induced by Paracoccidioides brasiliensis is associated with the non maturation of these cells

Abstract

Event Abstract Back to Event Inhibition of PGE2 and LTB4 production by dendritic cells induced by Paracoccidioides brasiliensis is associated with the non maturation of these cells Reginaldo K. Fernandes1*, Tatiana F. Bachiega1, Daniela R. Rodrigues1, Márjorie A. Golim2, Luciane A. Dias-Melicio3, Helanderson A. Balderramas1, Ramon Kaneno4 and Ângela M. Soares1* 1 Universidade Estadual Paulista "Júlio de Mesquita Filho" (UNESP) - Câmpus de Botucatu, Microbiologia e Imunologia - Instituto de Biociências, Brazil 2 Universidade Estadual Paulista "Júlio de Mesquita Filho" (UNESP), Hemocentro, Brazil 3 Universidade Estadual Paulista "Júlio de Mesquita Filho" (UNESP), Depto. Patologia - FMB - Botucatu, Brazil 4 Universidade Estadual Paulista "Júlio de Mesquita Filho" (UNESP), Microbiologia e Imunologia, Brazil The fungus Paracoccidioides brasiliensis is the etiological agent of paracoccidioidomycosis, a systemic mycosis endemic in Latin America. Dendritic cells (DCs) play a crucial role in the detection of pathogens, triggering an initial response from the host as well as the instruction of the adaptive immune response. Mediators released by DCs, by an autocrine way, modulate their functions. Among these, we highlight the prostaglandins and leukotrienes. We therefore assessed whether human DCs produce PGE2 and LTB4 in response to fungus, the participation of recognition receptors of molecular patterns (PRRs) in this production, as well as the modulating role of these eicosanoids on phenotypic maturation of these cells. Human immature DCs (CD14-/ CD1ahigh/CD83low) derived from the differentiation of monocytes cultured with GM-CSF and IL-4 (7 days) released substantial concentrations of PGE2 and LTB4 which increased significantly in the case of PGE2, with incubation with LPS for 1h, 2h, 4h, 8h, 12h, 18h, 24h or 48h. However, the levels of PGE2 and LTB4 were significantly inhibited after challenge with two different strains of the fungus. Assays using monoclonal antibodies to block PRRS showed the involvement of mannose receptor in PGE2 inhibition by the fungus. Additionally, phenotyping assays showed that after challenge with the fungus, DCs did not change their phenotype of immature cells to mature ones. Accordingly, we detected a clear association between inhibition of the production of PGE2 and LTB4 fungus and inability to induce maturation of DCs. Assays using exogenous PGE2 and LTB4 confirmed this association. Acknowledgements Financial Support: FAPESP 2011/12477-0 / 2011/04556-8 Keywords: Eicosanoids, Dendritic Cells, prostaglandin, leukotriene, Paracoccidioides brasiliensis Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Host-pathogen interactions Citation: Fernandes RK, Bachiega TF, Rodrigues DR, Golim MA, Dias-Melicio LA, Balderramas HA, Kaneno R and Soares ÂM (2013). Inhibition of PGE2 and LTB4 production by dendritic cells induced by Paracoccidioides brasiliensis is associated with the non maturation of these cells. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00536 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 25 Apr 2013; Published Online: 22 Aug 2013. * Correspondence: Mr. Reginaldo K Fernandes, Universidade Estadual Paulista "Júlio de Mesquita Filho" (UNESP) - Câmpus de Botucatu, Microbiologia e Imunologia - Instituto de Biociências, Botucatu-SP, Brazil, regiskeller@msn.com Prof. Ângela M Soares, Universidade Estadual Paulista "Júlio de Mesquita Filho" (UNESP) - Câmpus de Botucatu, Microbiologia e Imunologia - Instituto de Biociências, Botucatu-SP, Brazil, acsoares@ibb.unesp.br Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Reginaldo K Fernandes Tatiana F Bachiega Daniela R Rodrigues Márjorie A Golim Luciane A Dias-Melicio Helanderson A Balderramas Ramon Kaneno Ângela M Soares Google Reginaldo K Fernandes Tatiana F Bachiega Daniela R Rodrigues Márjorie A Golim Luciane A Dias-Melicio Helanderson A Balderramas Ramon Kaneno Ângela M Soares Google Scholar Reginaldo K Fernandes Tatiana F Bachiega Daniela R Rodrigues Márjorie A Golim Luciane A Dias-Melicio Helanderson A Balderramas Ramon Kaneno Ângela M Soares PubMed Reginaldo K Fernandes Tatiana F Bachiega Daniela R Rodrigues Márjorie A Golim Luciane A Dias-Melicio Helanderson A Balderramas Ramon Kaneno Ângela M Soares Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.