Anti-DEC-205 receptor carrying P10 induces protective response in experimental Paracoccidioidomycosis

Abstract

Event Abstract Back to Event Anti-DEC-205 receptor carrying P10 induces protective response in experimental Paracoccidioidomycosis Suelen S. Dos Santos1*, Eline V. Rampazo1, Karen S. Ferreira1, 2, Carlos P. Taborda1, Silvia B. Boscardin1 and Sandro R. Almeida1 1 University of São Paulo, Brazil 2 Unifesp, Brazil Paracoccidioidomycosis is a systemic mycosis caused by "Paracoccidioides brasiliensis", a thermally dimorphic fungus. It occurs in Latin America and is the most frequent endemic systemic mycosis in many countries of South America, where almost 10 million people are believed to be infected. In Brazil, it is ranked as the tenth cause of death among chronic infectious and parasitic diseases. It is likely that vaccines will need to elicit strong T cell-mediated immunity composed of IFNγ secreting CD4+ helper and CD8+ cytolytic T lymphocytes. To induce such responses, it would be valuable to harness the dendritic cell (DC) system of antigen-presenting cells. To assess the potential of targeting P10, a peptide derived from gp43 secreted by the fungus, directly to DCs, we cloned its sequence in fusion with a monoclonal antibody to the DEC-205 receptor, an endocytic receptor abundant on these cells in lymphoid tissues. We verified that αDEC/P10 antibody response induced large IFNγ-producing cells after single dose administration in relation to P10, even being administered at a lower concentration. However, this response was not maintained after the second dose of the antibody. After challenge of animals immunized with two doses of the antibody, we detected high levels IFNγ and IL-4 in the lung tissue was statistically higher in αDEC/P10 and ISO/P10 compared to administration of P10 and control Poly I:C. The results obtained indicate that targeting of P10 through αDEC/P10, in the presence of Poly I:C, is a promising strategy for vaccination against "P. brasiliensis". Acknowledgements Supported: FAPESP and CNPq. Keywords: "Paracoccidioides brasiliensis", Dendritic Cells, DEC205, Vaccination, Immunotherapy Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Translational immunology and immune intervention Citation: Dos Santos SS, Rampazo EV, Ferreira KS, Taborda CP, Boscardin SB and Almeida SR (2013). Anti-DEC-205 receptor carrying P10 induces protective response in experimental Paracoccidioidomycosis. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00815 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 18 Jun 2013; Published Online: 22 Aug 2013. * Correspondence: Ms. Suelen S Dos Santos, University of São Paulo, São Paulo, Brazil, suelen.silvana.santos@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Suelen S Dos Santos Eline V Rampazo Karen S Ferreira Carlos P Taborda Silvia B Boscardin Sandro R Almeida Google Suelen S Dos Santos Eline V Rampazo Karen S Ferreira Carlos P Taborda Silvia B Boscardin Sandro R Almeida Google Scholar Suelen S Dos Santos Eline V Rampazo Karen S Ferreira Carlos P Taborda Silvia B Boscardin Sandro R Almeida PubMed Suelen S Dos Santos Eline V Rampazo Karen S Ferreira Carlos P Taborda Silvia B Boscardin Sandro R Almeida Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.