IL-18 does not modulate dectin-1 expression on human monocytes challenged with Paracoccidioides brasiliensis

Abstract

Event Abstract Back to Event IL-18 does not modulate dectin-1 expression on human monocytes challenged with Paracoccidioides brasiliensis Muriel B. Cestari1, Tatiana F. Bachiega2, Reginaldo K. Fernandes2, Juliana C. De Quaglia E Silva1, Marjorie A. Golim3, Angela M. Soares2 and Luciane A. Dias-Melicio1* 1 Universidade Estadual Paulista "Júlio de Mesquita Filho" - UNESP, Depto. Patologia - Faculdade de Medicina de Botucatu, Brazil 2 Universidade Estadual Paulista "Júlio de Mesquita Filho" - UNESP, Depto. Microbiologia e Imunologia - Instituto de Biociências de Botucatu, Brazil 3 Universidade Estadual Paulista "Júlio de Mesquita Filho" - UNESP, Hemocentro - Faculdade de Medicina de Botucatu, Brazil IL-18 is a proinflammatory cytokine and its role in paracoccidioidomycosis, a deep mycosis caused by Paracoccidioides brasiliensis (Pb) is not fully understood. Recently, we demonstrated that this cytokine, by modulating TLR4 and MR expression, play an important role on human monocytes response to P. brasiliensis. The aim of this study was first to evaluate the involvement of dectin-1 on IL-18, TNF-α and IL-10 production by human monocytes in response to P. brasiliensis. Moreover, we asked whether IL-18 can modulate the production of these cytokines by altering the expression of this receptor. Monocytes were stimulated with IL-18 and treated with specific neutralizing antibody anti-dectin-1 before challenge with more virulent (Pb18) and less virulent strain (Pb 265) of the fungus . After, dectin-1 expression was analyzed by flow cytometry and IL-18, IL-10 and TNF-α production by ELISA. Results showed that higher levels of IL-18 and IL-10 were detected after challenge with Pb 18, in comparison to Pb 265. By other hand, Pb 265 strain induced higher levels of TNF-α. However, only TNF-α induced by the strains were decreased after blocking dectin-1 receptor, showing the involvement of this receptor only in the production of this cytokine. Moreover, cells preincubation with IL-18 did not affect cytokines production by monocytes in response to P. brasiliensis, as well as dectin-1 expression. We can hypothesize that at least in relation to dectin-1 receptor expression, lack of modulation by IL-18 could contribute to susceptibility of the response against the fungus. Financial Support: FAPESP 2012/01836-2. Acknowledgements Financial Support: FAPESP 2012/01836-2. Keywords: IL-18, dectin-1, Monocytes, Paracoccidioides brasiliensis, Cytokines Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Host-pathogen interactions Citation: Cestari MB, Bachiega TF, Fernandes RK, De Quaglia E Silva JC, Golim MA, Soares AM and Dias-Melicio LA (2013). IL-18 does not modulate dectin-1 expression on human monocytes challenged with Paracoccidioides brasiliensis. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00572 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 25 Apr 2013; Published Online: 22 Aug 2013. * Correspondence: Prof. Luciane A Dias-Melicio, Universidade Estadual Paulista "Júlio de Mesquita Filho" - UNESP, Depto. Patologia - Faculdade de Medicina de Botucatu, Botucatu, São Paulo, 18618-970, Brazil, ladiasmelicio@ibb.unesp.br Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Muriel B Cestari Tatiana F Bachiega Reginaldo K Fernandes Juliana C De Quaglia E Silva Marjorie A Golim Angela M Soares Luciane A Dias-Melicio Google Muriel B Cestari Tatiana F Bachiega Reginaldo K Fernandes Juliana C De Quaglia E Silva Marjorie A Golim Angela M Soares Luciane A Dias-Melicio Google Scholar Muriel B Cestari Tatiana F Bachiega Reginaldo K Fernandes Juliana C De Quaglia E Silva Marjorie A Golim Angela M Soares Luciane A Dias-Melicio PubMed Muriel B Cestari Tatiana F Bachiega Reginaldo K Fernandes Juliana C De Quaglia E Silva Marjorie A Golim Angela M Soares Luciane A Dias-Melicio Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.