Identification of NETs (neutrophil extracellular traps) in tegumentary lesions of patients with paracoccidioidomycosis

Abstract

Event Abstract Back to Event Identification of NETs (neutrophil extracellular traps) in tegumentary lesions of patients with paracoccidioidomycosis Amanda M. Della Coletta1*, Tatiana F. Bachiega2, Juliana C. De Quaglia E Silva1, Silvio A. Marques3, Mariângela E. Marques1, Julio Defaveri1, Angela M. Soares2 and Luciane A. Dias-Melicio1* 1 Universidade Estadual Paulista "Júlio de Mesquita Filho" - UNESP, Depto. Patologia, Brazil 2 Universidade Estadual Paulista "Júlio de Mesquita Filho" - UNESP, Depto. Microbiologia e Imunologia, Brazil 3 Universidade Estadual Paulista "Júlio de Mesquita Filho" - UNESP, Depto. Dermatologia e Radioterapia, Brazil Paracoccidiodomycosis is a systemic mycosis caused by Paracoccidioides brasiliensis (Pb), which is endemic in Latin America. In recent years, works in paracoccidioidomycosis have focused the role of neutrophils (PMNs), since the literature has demonstrated the dynamic involvement of these cells in host defense against various microorganisms. Recent studies have shown that neutrophils may use a third strategy to destroy microorganisms called NETosis, a type of neutrophil death, reported to be distinct from apoptosis, phagocytosis-induced cell death, and necrosis. This cell death pathway involves the release of extracellular traps by activated neutrophils, known as neutrophil extracellular traps (NETs). The NETs are composed by fibers containing decondensed chromatin, histones and several other granular proteins such as elastase, which in addition to ensnare and/or kill microorganisms, increase the inflammatory response. It has been proposed that NETs destroy microorganisms that were not phagocytosed by neutrophils, and this could be proposed for the Paracoccidioides brasiliensis, since the fungus yeast can present various sizes and morphologies. In this context, the objective of this study was identify the presence of NETs in vivo, analyzing tegumentary lesions of patients with paracoccidioidomycosis. Tissue section from formalin-fixed biopsies were stained with DAPI, anti-elastase (FITC) and anti-histone (Texas-Red); and analyzed by confocal imunofluorescence microscopy. The images revealed the presence of NETs constituents, nuclear and extracellular localization of DNA stained with DAPI, co-localization of elastase and histone, which was confirmed by the overlay of these 3 stains. Thus, we identified the mechanism of NETosis in paracoccidioidomycosis patient lesions. Financial Support: FAPESP/CNPq/Fundunesp Acknowledgements Financial Support: FAPESP 2011/18855-7; CNPq 480486/2011-5; Fundunesp 00637/11-DFP Keywords: NETs, Paracoccidioidomycosis, tegumentary lesions, Neutrophils, Paracoccidioides brasiliensis Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Host-pathogen interactions Citation: Della Coletta AM, Bachiega TF, De Quaglia E Silva JC, Marques SA, Marques ME, Defaveri J, Soares AM and Dias-Melicio LA (2013). Identification of NETs (neutrophil extracellular traps) in tegumentary lesions of patients with paracoccidioidomycosis. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00508 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 25 Apr 2013; Published Online: 22 Aug 2013. * Correspondence: Miss. Amanda M Della Coletta, Universidade Estadual Paulista "Júlio de Mesquita Filho" - UNESP, Depto. Patologia, Botucatu, São Paulo, 18618-970, Brazil, amdcoletta@gmail.com Prof. Luciane A Dias-Melicio, Universidade Estadual Paulista "Júlio de Mesquita Filho" - UNESP, Depto. Patologia, Botucatu, São Paulo, 18618-970, Brazil, ladiasmelicio@fmb.unesp.br Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Amanda M Della Coletta Tatiana F Bachiega Juliana C De Quaglia E Silva Silvio A Marques Mariângela E Marques Julio Defaveri Angela M Soares Luciane A Dias-Melicio Google Amanda M Della Coletta Tatiana F Bachiega Juliana C De Quaglia E Silva Silvio A Marques Mariângela E Marques Julio Defaveri Angela M Soares Luciane A Dias-Melicio Google Scholar Amanda M Della Coletta Tatiana F Bachiega Juliana C De Quaglia E Silva Silvio A Marques Mariângela E Marques Julio Defaveri Angela M Soares Luciane A Dias-Melicio PubMed Amanda M Della Coletta Tatiana F Bachiega Juliana C De Quaglia E Silva Silvio A Marques Mariângela E Marques Julio Defaveri Angela M Soares Luciane A Dias-Melicio Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.