Influences of fentanyl and sufentanil on the levothyroxine sodium preconditioning-induced cardioprotection in rats

Abstract

Objective To evaluate influences of fentanyl and sufentauil on the cardioprotection effect of preemptive levothyroxine-sodium administration in the rats. Methods Immature rats were randomly allocated into seven groups (n=8):blank control group (group BC),control group (group C),levothyroxine-sodium 10 μg group(group 10 μg),fentanyl group (group F),sufentanil group (group S),combined fentanyl and levothyroxine-sodium group (group F+L) and combined sufentanil and levothyroxine-sodium group(group S+L).The isolated heart model was established with a Langendorff apparatus.Hearts in the groups F and F+L were perfused with KH fluid containing fentanyl 30 μg/L,and hearts in the groups S and S+L were perfused with KH fluid containing sufentanil 3 μg/L. Results The hemodynamic variables at 30 min of the reperfusion were inferior in group F and S than in group BC,but better in group 10 μg,group F+L and group S+L than in group C,F and S.The coronary perfusion flows in group BC,group C,group 10 μg,group F,group S,group F+L and group S+L were (19±2),(16±1),(21±2),(17±1),(17±1),(21±1),(22±1) ml/min,respectively,with a significantly higher coronary perfusion flow in group 10 μg,group F+L and group S+L than in group C,group F and group S.The myocardial bound creatine kinase (CK-MB) level in the coronary perfusion fluid during the reperfusion period was significandy higher in groups C,F and S compared with groups 10 μg,F+L and S+L.The expression of b oth heat shock proteins70(HSP70) and myosin heavy-chain(MHC) mRNA in the myocardium was significantly higher in groups 10 μg,F+L and S+L than in groups C,F and S.However,there were no differences in all the observed parameters anong groups 10 μg,F+L and S +L. Conclusions Both fentanyl and sufentanil do not produce significant influence on the cardioprotective effect of levothyroxine sodium preconditioning. Key words: Fentanyl; Sufentanil; Preconditioning; Thyroid hormones; Cardioprotection