Abstract
Barley seeds were treated with ethyl methanesulphonate (EMS) for 5 h, washed for 18 h, exposed for 3 h to caffeine, re-dried to 30% water content and either immediately sown or stored for 10 days at 25°. The caffeine post-treatment considerably potentiated the EMS-induced M1 germination and the M1 seedling height reduction, but had no influence on the EMS-induced M1 sterility or percentage of M2 chlorophyll mutants. The caffeine post-treatment also had no significant influence on the “storage” recovery from the EMS-induced genetic effects.
Published Version
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