Abstract
Barley seeds were treated with ethyl methanesulphonate (EMS), washed for 24 h, redried to 15 per cent moisture and stored at 25°C. The criteria used for expressing the effect of storage were chromosomal aberrations in root tips, M1 germination, M1 seedling height, M1 survival and the frequency of M2 chlorophyll mutants. The increase of the M1 biological injury due to storage was not influenced: a) by applying EMS solutions at pH 2, pH 7 and pH 10, b) by lowering the EMS concentration and increasing the treatment time, c) by different variations of washing with water and by washings with 0.005 N NaOH, 200 mM cysteine or 200 mM thiourea. The rate of the increase of the M1 injury due to storage depends on the EMS dose. With a decrease in the EMS dose the storage effect is more delayed.
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