Abstract
Barley seeds were treated with propyl methanesulfonate (PMS) or isopropyl methanesulfonate (iPMS), washed for 0–24 hr and sown wet or redried to 15 per cent moisture and stored at 25 °C. The criteria used for expressing the influence of post-treatment washing were M1 seedling height, the frequency of chromosome aberrations in root tips, M1 germination, M1 survival, M1 fertility and the frequency of M2 chlorophyll mutants. (1) With increasing post-treatment washing time the mutagenic effects of PMS and iPMS considerably decreased. (2) After iPMS treatment the seeds can be redried to 15 per cent moisture without a post-treatment washing and show no increase of damage in contrast to PMS treated seeds where a 24 hr washing has to be applied to eliminate the effect of redrying. (3) With increasing post-treatment washing the potentiation of injury due to storage of PMS-treated seeds is more delayed. Storage of iPMS-treated seeds without washing resulted in a decrease of the induced mutagenic effects.
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